Salamander

  • 391 Posts
  • 561 Comments
Joined 4 years ago
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Cake day: December 19th, 2021

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  • I don’t have the Heltec V3, but the model I was able to find online does support WiFi.

    You need to connect to it using some other device. It can be with Bluetooth using an app, or via USB using a web client. Once you are connected to it, you can go to Radio Configuration and add the WiFi info in the Network settings.

    Then, you need to figure out what its IP is. There are a few different ways to do it. Usually I log into my router and check the IP of the latest device to log into the WiFi.

    You can then go to that IP via your browser, and you will see this:

    You can then connect to it and interact with the device over the browser.

    To interact with it using the python library, you set the device’s IP as the NODE’s interface. Here is an example of a very simple script that will connect to the node via its IP and cause it to automatically respond with ‘Pong’ to any message that it receives that contain ‘Ping’.

    import meshtastic
    import meshtastic.tcp_interface
    from pubsub import pub
    import time
    
    # Your Meshtastic node's IP
    MESHTASTIC_NODE_IP = "192.168.8.149"
    
    def onReceive(packet, interface):
        if 'decoded' in packet and 'payload' in packet['decoded']:
            try:
                message = packet['decoded']['payload'].decode('utf-8')
                sender = packet['from']
                if "Ping" in message:
                    interface.sendText("Pong", destinationId=sender)
            except Exception as e:
                pass
    
    interface = meshtastic.tcp_interface.TCPInterface(hostname=MESHTASTIC_NODE_IP)
    pub.subscribe(onReceive, "meshtastic.receive")
    
    while True:
        time.sleep(5)  # Keep the script running
    
    



  • I would give the Letharia dye another try

    Would love to… When I was in Oregon this lichen was super abundant. At the moment I am living in Amsterdam (Netherlands), and I see mostly Xanthoria, Evernia, Rhizocarpon, and a few other lichen species that grow on city trees, but they are very small and spotty, nothing compared to the wolf lichen in Oregon. I do miss the Oregon forests with the old growth sequoia redwood trees and all that lichen.


  • 9ft of snow?! I only experienced such deep snow in an urban setting while living in Connecticut for a year. I spent a few years in Oregon but the snow in the area never got so deep while I was there. When I was in the US I was not yet able to identify many fungi as I was mainly obsessed with animals (especially salamanders) back then, so unfortunately I did not really appreciate the diversity of fungi there. Although once in Oregon I did attempt to dye some socks using a wolf lichen (Letharia vulpina) and a pressure cooker. That did not end well.



  • Cool! I just read their wiki page and it says

    A snowbank fungus, it is most common at higher elevations after snowmelt in the spring.

    Snowbank fungus is a new term for me. Not sure yet what makes a fungus thrive through snow. Maybe they have anti-freeze proteins?

    Does your area get a lot of snow?





  • Alright! Some other tips:

    • Your current microscope is a 160 mm system, so make sure that the objectives are 160 mm and not infinity.
    • Make sure the objectives have an RMS thread
    • Once you move into higher-end objectives, you will have objectives that are specialized. For example, ‘phase contrast’ objectives have a dark ring inside of them. For the olympus brand their name often ends in ‘PL’. These work with bright-field too. My 40/1.30 objective is actually a phase-contrast objective because I did not know this and ChatGPT told me it meant something different 😂 However, the objective does work well for me and I am now considering upgrading to a phase contrast-capable microscope (the BH2), so I made a good choice by accident.








  • Always happy to talk about molecules interacting with light! 😄

    This is an aspect of lichen I hadn’t really put much thought into before now.

    I have some background is in studying how light interacts with molecules, so I probably put more thought and emphasis on these things than average.

    Its been in storage for a couple of months so I may try to re-hydrate it a bit before lighting it up during the night.

    That’s cool! When keeping a collection, can you keep them alive for a long time dry?



  • Not sure how I managed to never hit this species with UV. I would describe the colour as a bright, hot, lipstick pink. I am unsure if this lichen is actually fluorescing or if something else to do with how the pigments show up under UV light - maybe @Sal@mander.xyz would know. Picture doesn’t quite do it justice.

    You are pointing a UV lamp at it which probably sends out 365 nm or 395 nm photons. The lichen is shooting back photons with a broad range of wavelengths, and a lot of ~600 - 750 nm ones (red). So, the UV photons had to be “captured” by some molecular system, the system dissipated some energy, and then re-radiated some of these longer-wavelength photons.

    The general term that covers the many different possibilities is “photoluminescence”. In this case we can say for sure that the lichen exhibits “UV-induced photoluminescence”, because it is re-emitting lower energy (longer-wavelength) photons. It is common to make the connection “photoluminescence” = “fluorescence”, but technically fluorescence makes specific claims about how the light is re-emitted (singlet -> singlet emission), and it is not the only luminescence process. Other examples of luminescence are phosphorescence from a triplet state and luminescence via charge-recombination. So, to call it “fluorescence” in the strict sense we need know what the exact pathway is.

    That said, when it comes to biological pigments fluorescence is generally the most common pathway. Triplets that live long enough to produce light are generally undesirable as they can react indiscriminately with molecules inside of the cell as well as produce reactive oxygen species, and good phosphorescent materials often combine metals and heavy atoms that are not as abundant in living tissue.

    So, knowing nothing else, and seeing that red light comes out when you shine UV/blue light on a lichen, it is generally fair to call it “fluorescence”.

    Now, if we discuss this specific lichen… I have looked it up and it does get interesting! Do you have it with you? I suspect that its fluorescence might be different during the day than during the night.

    I can find online two significant fluorescent components: parietin, which produces the fluorescent yellow pigment, and Chlorophyll a/b, which produces red fluorescence. There is an interesting paper exploring the idea that one functional purpose of parietin’s fluorescence is that it can transfer energy to the algae to boost their photosynthesis. Their conclusions in the paper is that the idea is not supported by the evidence, so, a “negative result”. It is a fun example of the type of research that is performed in photobiology and also an example to show that even negative results can be interesting enough to be published!

    As for the difference between day and night - if what you see is a combination of the fluorescence of parietin and chlorophyll, then the color might change with the day/night cycle. Photosynthetic organisms regulate the flow of excess photon energy towards a safe non-radiative dissipation pathway in response to light. This is called the non-photochemical quenching pathway, and during the day this pathway tends to be active. During the night there is little light, and so this protective pathway shuts-off. This allows more of the absorbed photon energy to flow into the radiative fluorescence pathway, increasing the red fluorescence. You can actually see this easily with plants - you can dark adapt a leaf and then compare its fluorescence with that of a leaf that is being exposed to a bright light. The dark-adapted one will usually show significantly more red fluorescence.

    This time you did ask, so I won’t apologize for my essay 😆 But I am a bit sorry I didn’t have the time to make it shorter.